Vol. 34 (2024)
Artículos de Investigación

Optimización y caracterización de lipasas tolerantes a solventes de Geobacillus stearothermophilus CHI1

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  • César Octavio García Hernández,
  • Lucía María Cristina Ventura Canseco,
  • Miguel Abud Archila,
  • Sandy Luz Ovando Chacón,
  • Peggy Elizabeth Alvarez Gutiérrez
César Octavio García Hernández
Instituto Tecnológico de Tuxtla Gutiérrez-Tecnológico Nacional de México
Lucía María Cristina Ventura Canseco
Instituto Tecnológico de Tuxtla Gutiérrez-Tecnológico Nacional de México
Miguel Abud Archila
Instituto Tecnológico de Tuxtla Gutiérrez-Tecnológico Nacional de México
Sandy Luz Ovando Chacón
Instituto Tecnológico de Tuxtla Gutiérrez-Tecnológico Nacional de México
Peggy Elizabeth Alvarez Gutiérrez
CONAHCYT-Tecnológico Nacional de México-Instituto Tecnológico de Tuxtla Gutiérrez
DOI: https://doi.org/10.15174/au.2024.4153

Publicado 2024-07-24

Cómo citar

García Hernández, C. O., Ventura Canseco, L. M. C., Abud Archila, M., Ovando Chacón, S. L. ., & Alvarez Gutiérrez, P. E. (2024). Optimización y caracterización de lipasas tolerantes a solventes de Geobacillus stearothermophilus CHI1. Acta Universitaria, 34, 1–13. https://doi.org/10.15174/au.2024.4153

Resumen

Las lipasas son enzimas atractivas para su uso en biotecnología, principalmente aquellas producidas por extremófilos. Recientemente fueron aisladas bacterias termófilas Geobacillus en aguas geotermales del lago del cráter del volcán “El Chichón”, en México. En este trabajo se caracterizó y optimizó la actividad lipolítica de la cepa Geobacillus stearothermophilus CHI1. Esta cepa fue capaz de producir enzimas con máxima actividad lipolítica a 60 °C y 80 °C, con valores de pH de 5, 9 y 11, además de que demostró ser tolerante a solventes y capaz de realizar catálisis independientemente de iones metálicos. Adicionalemente, mostró mayor afinidad hacia sustratos de cadena media. Los resultados de la caracterización y optimización bioquímica sugieren la presencia de más de un tipo de actividad lipolítica presente en Geobacillus stearothermophilus CHI1. Todas estas características hacen que estas enzimas sean atractivas en procesos biotecnológicos a altas temperaturas y pH alcalino (por ejemplo, aditivos detergentes), así como para ayudar a comprender su utilidad biológica en Geobacillus.

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Citas

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